Applied Biosystems 7500 - Chapter 3 Prepare the Reactions

Chapter 3
43
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Notes
Prepare the Reactions
This chapter covers:
■ Chapter Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
■ Prepare the Sample Dilutions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
■ Prepare the Standard Dilution Series . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
■ Prepare the Reaction Mix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
■ Prepare the Reaction Plate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
Note: For more information about any of the topics discussed in this guide, open the
Help from within Applied Biosystems 7500/7500 Fast Real-Time PCR Software v2.0 by
pressing F1, clicking in the toolbar, or selecting Help7500 Software Help.