Chapter 5 Analyze the Experiment
View the Standard Curve
71
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Notes
View the Standard Curve
The Standard Curve screen displays the standard curve for samples designated as
standards. The 7500 software calculates the quantity of an unknown target from the
standard curve.
About the
Example
Experiment
In the standard curve example experiment, you review the Standard Curve screen for the
following regression coefficient values:
• Slope/amplification efficiency
•R
2
value (correlation coefficient)
•C
T
values
Note: In the example experiment, the slope, R
2
, and amplification efficiency values are
affected by the C
T
of well B12 which you will omit later in this guide.
View the
Standard Curve
1. In the navigation pane, select Analysis Standard Curve.
Note: If no data are displayed, click Analyze.
2. Display all 96 wells in the Standard Curve screen by clicking the upper left corner
of the plate layout in the View Plate Layout tab.
3. In the Target drop-down list, select All.
4. In the Plot Color drop-down list, select Default.
5. Click (Show a legend for the plot).
Note: This is a toggle button. When the legend is displayed, the button changes to
Hide the plot legend.
6. View the values displayed below the standard curve. In the example experiment, the
values for the target (RNase P) are within the acceptable ranges:
– The slope is -3.685.
–The R
2
value is 0.992.
– The amplification efficiency (Eff%) is 86.81%.
To Next Page
Loading...
