Applied Biosystems 7500 - Define the Methods and Materials

Chapter 2 Design the Standard Curve Experiment
Define the Methods and Materials
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
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Notes
Define the Methods and Materials
In the Methods & Materials screen, select the quantitation method, reagents, ramp speed,
and PCR template to use for the experiment.
About the
Example
Experiment
In the standard curve example experiment:
• The standard curve quantitation method is used.
•TaqMan
®
reagents are used.
• The standard ramp speed is used in the instrument run.
• Purified gDNA (isolated from two populations) is the template type. You must first
extract the gDNA from tissue or sample.
Complete the
Methods &
Materials Screen
1. Select Standard Curve as the quantitation method.
2. Select TaqMan
®
Reagents for the reagents.
3. Select Standard (~ 2 hours to complete a run) for the ramp speed.
4. Select gDNA (genomic DNA) for the template type.
5. Click Next.
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