Chapter 2 Design the Standard Curve Experiment
Chapter Overview
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
18
Notes
Chapter Overview
This chapter explains how to use the Design Wizard in the 7500 software to set up the
standard curve example experiment. The Design Wizard guides you through Applied
Biosystems recommended best practices as you enter design parameters for the example
experiment.
Note: When you design your own experiments, you can select alternate workflows (see
“Using This Guide with Your Own Experiments” on page 11).
Example
Experiment
Workflow
Analyze the Experiment (Chapter 5)
Start Experiment
Prepare the Reactions (Chapter 3)
Run the Experiment (Chapter 4)
End Experiment
Design the Experiment (Chapter 2)
1. Create a new experiment.
2. Define the experiment properties.
3. Define the methods and materials.
4. Set up the targets.
5. Set up the standards.
6. Set up the samples.
7. Set up the run method.
8. Review the reaction setup.
9. Order materials for the experiment.
10. Finish the Design Wizard.
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