Applied Biosystems 7500 - Performing the Background Calibration and Optical Calibration; Preparing the Background Calibration Plate; Creating a Plate Document for the Background Calibration; Performing the Background Calibration

Chapter 3 Prepare the Reactions
Prepare the Reaction Plate
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Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Notes
Prepare the Reaction Plate
Prepare the reactions for each replicate group, then transfer them to the reaction plate
using the plate layout displayed in the 7500 software.
About the
Example
Experiment
For the standard curve example experiment:
•A MicroAmp
®
Optical 96-Well Reaction Plate is used.
• The reaction volume is 25 µL/well.
• The reaction plate contains:
– 6 Unknown wells
– 15 Standard wells
– 3 Negative control wells
– 72 Empty wells
• The plate layout that the 7500 software automatically generates is used: