Applied Biosystems 7500 - Index

123
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Index
Numerics
1-step RT-PCR 8, 23, 24, 31, 36
2-step RT-PCR 8
, 23, 24
7500/7500 Fast system
consumables 4
data collection 2
filters 3
, 95
reagents 9
A
Advanced Setup 10, 11, 23, 29, 53, 98
alternate experiment workflows. See workflows.
amplification efficiency 27
, 73
Amplification Plot screen
monitor during a run 62
view after a run 74
amplification plot, typical 78
AMPNC flag 89
analysis screens
Amplification Plot screen 74
Multicomponent Plot screen 92
Multiple Plots screen 70
navigation tips 69
QC Summary screen 88
Raw Data Plot screen 94
Standard Curve screen 71
Well Table 81
analysis settings
advanced 87
baseline 87
CT 87
flag 87
threshold 87
view 86
analyze experiment
analyze 68
for more information 73
, 80, 83, 87, 89, 91, 94, 95
guidelines 69
, 73, 78, 83, 87, 89, 91, 94, 95
omit wells 90
publish the data 83
view Amplification Plot screen 74
view analysis settings 86
view Multicomponent Plot screen 92
view Multiple Plots screen 70
view QC Summary screen 88
view Raw Data Plot screen 94
view Standard Curve screen 71
view Well Table 81
workflow 66
Applied Biosystems
contacting xi
customer feedback on documentation xi
Technical Support xi
Applied Biosystems 7500/7500 Fast Real-Time PCR
System. See 7500/7500 Fast system.
assumptions for using this guide viii
B
BADROX flag 89
baseline
correct values 78
examples 80
manually adjust 87
biohazardous waste, handling xix
biological hazard guidelines xxi
BLFAIL flag 89
C
CAUTION, description xii
chemical safety xvii
, xviii
chemical waste safety xix
consumables
Also see materials required 4
supported 4
conventions
safety xii
conventions used in this guide viii
create new experiment 19
CT 83
CTFAIL flag 89
Custom assays 36
customer feedback, on Applied Biosystems
documents xi
D
DANGER, description xii
data
about data collection 2
example experiment 11
, 68
publish 83
design experiment
create new 19
define experiment properties 20
define methods and materials 22
finish Design Wizard 40