Applied Biosystems 7500 Getting Started

Chapter 5 Analyze the Experiment
Chapter Overview
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
66
Notes
Chapter Overview
The 7500 software analyzes the data using the standard curve quantitation method.
Section 1 of this chapter explains how to review the analyzed data using several of the
analysis screens and how to publish the data. If you obtain questionable results, Section 2
of this chapter explains how to perform some troubleshooting steps.
Example
Experiment
Workflow
Design the Experiment (Chapter 2)


Start Experiment


Prepare the Reactions (Chapter 3)
Run the Experiment (Chapter 4)

End Experiment
Analyze the Experiment (Chapter 5)
Section 1, Review Results:
1. Analyze.
2. View the standard curve.
3. View the amplification plot.
4. View the results in a table.
5. Publish the data.
Section 2, Troubleshoot (If Needed):
1. View the analysis settings; adjust the baseline/threshold.
2. View the quality summary.
3. Omit wells.
4. View the multicomponent plot.
5. View the raw data plot.

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)