Applied Biosystems 7500 - Prepare for the Run

Chapter 4 Run the Experiment
Prepare for the Run
57
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Notes
Prepare for the Run
Prepare for the run by opening the example experiment file you created in Chapter 2,
then loading the sealed reaction plate into the 7500/7500 Fast instrument.
Open the
Example
Experiment
1. Double-click (7500 software) or select StartAll Programs Applied
Biosystems7500 Software<software name>
where <software name> is the current version of the 7500 software.
2. In the Home screen, click Open.
3. In the Open dialog box, navigate to the experiments folder (default):
<drive>:\Applied Biosystems\<software name>\experiments
4. Double-click
Standard Curve Example Setup.eds to open the example experiment
file that you created in Chapter 2.
Load the
Reaction Plate
Into the
Instrument
PHYSICAL INJURY HAZARD. During operation, the sample
block can be heated to 100 °C. Before performing the following procedure, be sure to
wait until the sample block reaches room temperature.
IMPORTANT! Wear powder-free gloves when you handle the reaction plate .
1. Push the tray door to open it.
2. Load the plate into the plate holder in the instrument. Ensure that the plate is
properly aligned in the holder.
GR2477
7300/7500
barcode on plate
GR2477
7500 system
Load standard plates with
the notched A12 position at
the top-right of the tray.
7500 Fast system
Load Fast plates with the
notched A1 position at the
top-left of the tray.
GR2475
GR2475
7500
keyed corner
A
B
C
D
E
F
G
H
1 2 3 4 5 6 7 8 9 10 11 12
A
1
GR2475
GR2475
7500
keyed corner
A
B
C
D
E
F
G
H
1 2 3 4 5 6 7 8 9 10 11 12
A
1