Applied Biosystems 7500 - Page 82

Chapter 4 Run the Experiment
Prepare for the Run
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
58
Notes
3. Place the reactions in the precision plate holder (PPH). If you use:
• A reaction plate – Place the reaction plate in the PPH with well A1 at the
back-left corner.
Note: Fast Reaction 8-tube strips can be used only on the 7500 Fast system.
For the 7500 system, use MicroAmp
®
Optical 8-Tube Strips.
• Reaction tube strips – Place the tube strips in the PPH for tube strips.
Note: Fast Reaction tubes cannot be used in the 7500 Fast system.
• Reaction tubes – Place the tubes in the PPH.
Note: MicroAmp
®
Fast Reaction Tubes (PN 4358297) cannot be used in the
7500 Fast system.
IMPORTANT! For optimal performance with partial loads:
For a 7500 Instrument
Load at least 16 tubes, arranging them first in the center columns of the
instrument system (columns 6 and 7). Move outward towards columns 1 and 12
as you add more tubes.
For a 7500 Fast Instrument
• Place empty tube strips in columns 1 and 12 to prevent crushing of tubes
containing samples.
• Place tube strips with samples in the PPH vertically, starting in columns 6
and 7 and moving outward.
• A maximum of 6 tube strips can be used in the 7500 Fast instrument.
Leave columns 2, 3, 10, and 11 empty.
4. Close the tray door. Apply pressure to the right side of the tray and at an angle.
GR2471
GR2471
7500
tray top view
Push