Applied Biosystems 7500 - Purpose of RNase P Runs

Chapter 5 Analyze the Experiment
Omit Wells from the Analysis
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Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
Notes
6. Click Analyze to reanalyze the experiment data with the outlying well(s) removed
from the analysis.
7. Review the data analyzed without the outlier:
a. In the navigation pane, select Analysis Standard Curve.
b. Display all 96 wells in the Standard Curve screen by clicking the upper left
corner of the plate layout in the View Plate Layout tab.
c. In the Target drop-down list, select All.
d. In the Plot Color drop-down list, select Default.
8. View the values displayed below the standard curve. In the example experiment, the
values for the target (RNase P) are within the acceptable ranges:
– Slope is -3.614.
–R
2
value is 0.993.
– Amplification efficiency (Eff%) is 89.118%.
Analysis
Guidelines
When you analyze your own standard curve experiment, carefully view the replicate
groups for outliers. If needed, remove outliers manually using the Well Table.
For More
Information
For more information on omitting wells from the analysis, open the 7500 Software Help
by clicking or pressing F1. Within the Help, search for the omit well topics:
1. Select the Search tab.
2. Enter omit well.
3. Click List Topics.
4. Double-click the topics you want to review.