Applied Biosystems 7500 Getting Started

Preface
How to Obtain More Information
Applied Biosystems 7500/7500 Fast Real-Time PCR System Getting Started Guide for Standard Curve
Experiments
x
Documents Related to Standard Curve Experiments
Documents Related to the Reagent Guide
Note: For more documentation, see “How to Obtain Support” on page xi.
Document PN
Amplification Efficiency of TaqMan
®
Gene Expression Assays Application Note 127AP05
Custom TaqMan
®
Gene Expression Assays Protocol 4334429
Primer Express
®
Software Version 3.0 Getting Started Guide 4362460
TaqMa n
®
Gene Expression Assays Protocol 4333458
User Bulletin #2: Relative Quantitation of Gene Expression 4303859
Document PN
Applied Biosystems High-Capacity cDNA Reverse Transcription Kits Protocol 4375575
Custom TaqMan
®
Gene Expression Assays Protocol 4334429
Custom TaqMan
®
Genomic Assays Protocol: Submission Guidelines 4367671
Custom TaqMan
®
SNP Genotyping Assays Protocol 4334431
Power SYBR
®
Green PCR Master Mix and RT-PCR Protocol 4367218
Pre-Developed TaqMan
®
Assay Reagents Allelic Discrimination Protocol 4312214
Primer Express
®
Software Version 3.0 Getting Started Guide 4362460
SYBR
®
Green PCR and RT-PCR Reagents Protocol 4304965
SYBR
®
Green PCR Master Mix and RT-PCR Reagents Protocol 4310251
TaqMa n
®
Drug Metabolism Genotyping Assays Protocol 4362038
TaqMa n
®
Exogenous Internal Positive Control Reagents Protocol 4308335
TaqMa n
®
Fast Universal PCR Master Mix (2✕) Protocol 4351891
TaqMa n
®
Gene Expression Assays Protocol 4333458
TaqMa n
®
Gene Expression Master Mix Protocol 4371135
TaqMa n
®
Genotyping Master Mix Protocol 4371131
TaqMa n
®
SNP Genotyping Assays Protocol 4332856
TaqMa n
®
Universal PCR Master Mix Protocol 4304449
User Bulletin #2: Relative Quantitation of Gene Expression 4303859
Using TaqMan
®
Endogenous Control Assays to Select an Endogenous Control
for Experimental Studies Application Note
127AP08

Applied Biosystems 7500 Specifications

General

Detection Method	Fluorescence
Sample Capacity	96-well plate
Thermal Cycler	Yes
Temperature Uniformity	±0.5°C
Type	Real-Time PCR System
Thermal Accuracy	±0.25°C
Detection Channels	5
Weight	45 kg
Power Requirements	100-240 V, 50/60 Hz
Dynamic Range	9 logs
Reaction Volume Range	10-100 µL
Temperature Range	4°C to 100°C
Thermal Cycler Block	96-well
Software	SDS Software
Detection	Multiplex (up to 5 colors)