Chapter 5 Generating Data from RQ Plates – 7500 Fast System
Creating a Relative Quantification (RQ) Plate Document
Applied Biosystems 7300/7500/7500 Fast Real-Time PCR System Relative Quantification Getting Started Guide 47
Notes
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5
Sample Experiment
In the example RQ experiment, the samples for each of the three tissues (liver, kidney, and bladder) are loaded on three
separate plates. Consequently, three RQ Plate documents are created, one for each of the sample plates.
Because the experiment is singleplex, there is only one sample—either a target or endogenous control—in each well. Each
well is associated with a detector (indicated by the colored squares). Additionally, each well is assigned a detector task—
T (target) or E (endogenous control).
The figure below shows the example RQ Plate document after sample names, detectors, and detector tasks are assigned
for each well in the liver plate.
Sample name
Detector task and
color
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