136 BD FACSCanto Flow Cytometer Reference Manual
Excitation Optics
Emission Optics
Optical platform Fixed optical assembly
Beam geometry Blue and red laser: 9 µm x 65 µm elliptical beam
Collection lens Optical gel–coupled to flow cell
Numerical aperture (NA) = 1.2
Fluorescence detection 6 photomultiplier tube detectors:
Four wavelength ranges detected from 488-nm
laser:
• 750–810 nm (PE-Cy7)
• >670 nm (PerCP-Cy5.5)
• 564–606 nm (PE)
• 515–545 nm (FITC)
Two wavelength ranges detected from 633-nm
laser:
• 750–810 nm (APC-Cy7)
• 650–670 nm (APC)
Forward scatter detection Photodiode with 488/10 bandpass filter
Side scatter detection PMT with 488/10 bandpass filter
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