102 BD FACSCanto Flow Cytometer Reference Manual
Loader Troubleshooting
Observation Possible Causes Recommended Solutions
Tube does not load Loader not set up for
automatic loading
Set up the Loader according to
Changing to Automatic Loading
on page 41. Make sure both the
adapter lever and tube guide were
moved as shown.
Tube not completely raised Make sure the Loader drawer is
completely shut.
Make sure the tube is free of bulky
labels or tape.
Tube lifter hitting carousel
during ascent
Make sure the carousel is properly
engaged with the alignment guide
pin. If the problem persists,
contact BD Biosciences for
assistance.
Tube lifter failure Contact BD Biosciences.
Tube missed, or missing
tube messages in
software
Salt crystal buildup on optical
sensors
Clean the tube optical sensor
(outer sensor), as described in
Cleaning External Surfaces on
page 43.
Condensation on tube Wipe tube.
Tube label reflection Turn tube.
Tube runs dry, Loader
not advancing to next
sample
Dilute sample or rare events Set a stopping time in
BD FACSDiva software. Refer to
the BD FACSCanto Instructions
for Use.
Bubble in flow cell diverts
stream
Degas flow cell. See Removing
Bubbles from the Flow Cell on
page 76.
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