96 BD FACSCanto Flow Cytometer Reference Manual
Cytometer Troubleshooting
Observation Possible Causes Recommended Solutions
Flow cell will not fill Air in bubble filter Run the Bubble Filter Purge
command at least once (you might
need to run it several times).
When finished, run De-gas Flow
Cell. See Purging the Bubble Filter
on page 78.
Fluidics cart power off Turn on the power to the fluidics
cart by resetting the fluidics cart
circuit breaker switch (Figure 1-7
on page 27). Always use the
cytometer power button, located
on the left side of the cytometer, to
turn the system off and on.
No pressure in the plenum • Check air tubing connections.
• Check air tubing for kinks.
Sheath line disconnected
1 Check cubitainer-to-fluidics
cart, and fluidics cart–to-
cytometer connections. See
Reconnecting the Fluidics Cart
Tubing on page 85.
2 Check tubing for kinks.
BD FACSFlow cubitainer
empty
Replace the BD FACSFlow
cubitainer. See Changing a
Cubitainer on page 68.
Air in BD FACSFlow filter
(fluidics cart)
Purge air from the filter. See
Purging the Fluidics Filters on
page 57.
Air lock in BD FACSFlow
filter (on fluidics cart)
See Removing an Air Lock on
page 72.
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