Chapter 5: Troubleshooting 97
Flow cell will not fill
(continued)
No air pressure in cart • If the cart is attached to an
auxiliary air supply, switch on
auxiliary air.
• If the cart is not attached to an
auxiliary air supply, switch off
auxiliary air.
Fluidics pressure errors Air lock in filter • Check the filter in the fluidics
cart. Verify the bottom bleeder
valve on the filter is fully
tightened. Open the top bleeder
valve. If no fluid leaks out,
remove the air lock as
described in Removing an Air
Lock on page 72.
Fluid backfill into
sample tube
Cracked tube • Transfer sample to new tube.
• Make sure you are using
appropriate tubes. See System
Requirements on page 31.
Bal seal improperly installed
or worn
Reinstall or replace the Bal seal.
See Replacing the Bal Seal on
page 80.
Air lock in BD FACSFlow
filter
See Removing an Air Lock on
page 72.
Liquid leakage around
cytometer base
Interior valve failure
1 Turn off the cytometer power.
2 Clean up the liquid, using
proper precautions.
3 Call BD Biosciences.
QC fails after Long
Clean
Residual BD FACSClean
solution in lines
Run Fluidics Startup to flush the
system with sheath fluid. Repeat
until QC passes.
Cytometer Troubleshooting (continued)
Observation Possible Causes Recommended Solutions
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