80 BD FACSCanto Flow Cytometer Reference Manual
Decontaminating the Fluidics System for Storage
If your cytometer is to be taken out of operation, perform the following
procedure to clean out the fluidics lines and fill them with BD FACS shutdown
solution.
1 Perform steps 1 through 4 in Decontaminating the Fluidics System (Long
Clean) on page 59.
2 Run fluidics shutdown.
3 Shut down the software and turn off the power to the cytometer.
Replacing the Bal Seal
The Bal seal is a Teflon ring that allows the sample tube to pressurize. Over time,
this seal becomes worn or cracked and requires replacement. Replace the seal
when persistent sample tube pressure errors occur.
Over time, saline deposits may develop on the interior surface of the SIT Bal seal
retainer, causing an incomplete SIT flush between samples. Therefore, you should
always rinse the retainer in DI water when you replace a Bal seal.
Figure 4-5 Bal seal and retainer
Bal seal
retainer
SIT
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