BD FACSCanto - Replacing the Air Filter

60 BD FACSCanto Flow Cytometer Reference Manual
4 Click OK.
5 Select to shut down or continue.
• To shut down, select Cytometer > Fluidics Shutdown.
Repeat Fluidics Shutdown twice more.
Quit the software and turn off the power to the cytometer.
• To continue, select Cytometer > Fluidics Startup.
Repeat Fluidics Startup twice more.
BD FACS shutdown solution can lyse cells. The Fluidics Startup
procedure removes BD FACS shutdown solution from the interior
reservoir and fluid lines and replaces it with BD FACSFlow solution.
; Tip If setup fails after a long clean, repeat Fluidics Startup until setup
passes.
Replacing the Air Filter
The BD FACSCanto cytometer has an air filter located in the side door. Replace
the filter every 6 months to ensure proper cytometer performance.
Materials
Replacement filter (see Other Replacement Parts on page 129 for ordering)
Procedure
1 Turn off the cytometer power.
2 Open the side door.
• Press the black button; a handle will pop out.
If the completion message does not display after 90 minutes, verify there
are no error messages in the Status tab of the Cytometer window. If the
cleaning mode fails, see Fluidics Cart Troubleshooting on page 101.