66 BD FACSCanto Flow Cytometer Reference Manual
Unscheduled Maintenance
Perform these maintenance procedures as needed.
Procedure Description and Location Frequency
Replace cubitainers See Changing a Cubitainer on
page 68.
As needed
Prime fluid lines Removes air from fluid lines
See Priming Fluidics Lines on
page 71.
Whenever a fluidics line
is disconnected to change
a cubitainer or perform
other maintenance
Remove an air lock Removes air from filter and
restores flow of fluid
See Removing an Air Lock on
page 72.
When fluidics are not
functioning properly
(flow cell will not fill, or
there is backflush into
sample tube)
Clean external surfaces Keeps surfaces free from saline
deposit buildup after exposure
to sheath fluid
See Cleaning External Surfaces
on page 75.
As needed
De-gas Flow Cell Removes bubbles from the flow
cell
See Removing Bubbles from the
Flow Cell on page 76.
As needed during daily
startup
Clean Flow Cell Runs BD FACSClean solution
through the SIT and flow cell
See Cleaning the Flow Cell on
page 77.
When poor optical
performance indicates
additional cleaning is
needed
Bubble Filter Purge Removes air from the bubble
filter
See Purging the Bubble Filter on
page 78.
If fluidics run dry, or
when CVs are poor
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