BD FACSCelesta Flow Cytometer - Page 150

BD FACSCelesta Flow Cytometer User’s Guide150
O
online Help 11
optic holder 41
optics
components 28
configuration 64, 66
dichroic mirrors 108
filters 28, 104
optimization, sample 62
ordering spare parts 144
O-ring
ordering 146
replacing 58
worn 118
P
particles 144, 145
PerCP, Stokes shift 102
performance check 67, 68
photodiode 30
photomultiplier tubes (PMTs) 29
PMT See photomultiplier tubes
polygon
shown 29
population hierarchy 91
PRIME, fluid control button 21
priming fluidics system 40
propidium iodide (PI) 47
pulse
definition 112
measurements 113
processors 113
Q
quality control (QC)
particles 144
troubleshooting 125
R
recording
compensation settings 81
compensation tubes 80
data 86, 88
removing air bubbles, filter 36
replacing
Bal seal 56
sample tube O-ring 58
sheath filter 54
waste air filter 52, 60
reusing analyses 97
RUN
button orange 121
fluid control button 21
S
safety symbol definitions 11
safety symbols 11
sample
optimization 62
optimization experiment 72
optimization, single-stained
controls 63, 86
sample injection port (SIP)
components 25
hydrodynamic focusing 100
replacing Bal seal 56
replacing sample tube O-ring 58
sample tube
injection 25
not fitting 119
requirements 119
samples, running 88
saving analyses 97
scatter
light 102
parameter distortion 124
setup, cytometer 62