BD FACSCelesta Flow Cytometer - Page 149

Index 149
replacing 54
FITC and Stokes shift 103
fixed-alignment lasers 17
flow cell
draining 40
fluidics 100
flow rate control buttons 22
fluid control buttons
PRIME 21
RUN 21
STANDBY 21
fluidics
description 20, 100
flow rate control 22
flushing system 50
priming 40
removing air bubbles 36
run mode 21
sample injection port (SIP) 25
fluidics controls, cytometer 19
fluidics interconnect 37
fluorescence
defined 102
emission 112
fluorescence signal, none 121
fluorochromes
compensation theory 109
emission spectra 103
measured 42
role in light emission 102
flushing the system 50
forward scatter (FSC) 102
FSC See forward scatter
G
gating data 91
global worksheets
analyzing data 91
creating 87
previewing data 86, 97
H
hazard symbol definitions 11
Help, accessing 11
High Throughput Sampler (HTS) 26
hydrodynamic focusing 100
I
immunophenotyping
analysis 91
experiment 87
hydrodynamic focusing 101
L
lasers
options 28
performance check 67
quality control (QC) particles 144
longpass (LP) filters
defined 104
theory 105
LP See longpass filters
M
maintenance
Bal seal 56
cytometer shutdown 49
sample tube O-ring 58
schedule 47
scheduled 50
sheath filter 54
system flush 50
mirrors, dichroic longpass (LP) filter 29
N
notch filters 104