BD FACSCelesta Flow Cytometer - Page 148

BD FACSCelesta Flow Cytometer User’s Guide148
waste container shown 38
computer system, about 30
configuration
BD FACSDiva 64
containers
sheath 27, 33
waste 27, 38, 40
control panel, cytometer 19
controls
fluidics 19
single-stained 63, 86
conventions
safety symbols 11
creating
analysis objects 91
global worksheets 87
statistics view 91
CS&T particles 145
CV, troubleshooting 125
cytometer 17
configuration 42, 64
disconnect error 126
setup 62
starting 32
troubleshooting 118
D
Daily cleaning 47
DAPI, cleaning after using 47
data
analyzing 86, 91
gating 91
recording 86, 88
debris, excessive 124
detector arrays 28
detectors 30
dichroic mirrors
theory 108
digital data 17
digital electronics 114
Diva software See software
DNA, flow rate for analysis 101
droplet containment system 26
E
electronics
digital 114
laser controls 115
pulse 112
pulse measurements 113
threshold 114
troubleshooting 126
emission duration 112
event rate
erratic 123
high 122
low 122
zero 119, 121
excessive debris 124
excitation wavelength 102
experiments
creating 72
immunophenotyping 87
sample optimization 72
specifying parameters 73
F
FACSFlow sheath fluid 145
FACSFlow solution 35
Falcon tubes 119
filters, optical
bandpass (BP) 29, 107
dichroic See dichroic mirrors
longpass (LP) 104, 105
notch 104
shortpass (SP) 104, 106
theory 104
filters, sheath
attaching new 55