Getting Started Page 31 of 42
Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.
2. Enter the plate’s Serial Number.
3. Place the plate on the carrier. Tap Required (or Realign) and then Start to perform the
alignment. The process takes several minutes to complete.
4. Change the Blank threshold CV% if the default value does not meet your assay
requirements. This setting compares all replicates of a blank to determine cleanliness
of the microspots (dirty microspots can skew the %CV). Set the value smaller to have a
tighter tolerance on the deviation of cleanliness of the microspots, or set it higher to
relax the tolerance.
5. Tap Edit and enter the pathlength values that either came with the Take3 plate or
were generated through the Pathlength Correction procedure in the Take3 User
Guide.
6. When finished, tap Back and Home to return to the Main Menu.
Run a Take3 Protocol
1. On the Main Menu, tap the Take3 tab and select the protocol to run.
2. Select 1 to 12 wells as Blank and tap Continue.
3. On the Run Protocol screen, select options as applicable:
• Concentration units: Select the unit of measure for the export file.
• Secondary Ratio WL (230 nm): Available for nucleic acid protocols.
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