Getting Started Page 32 of 42
Epoch 2 Instructions for Use 1771011 Revision A BioTek Instruments, Inc.
• Spectral Scan: Perform a spectral scan for the wavelength range displayed (1
nm step size), in addition to the endpoint reads.
.
4. Place the Take3 plate on the carrier and tap Start.
• If the Take3 Blank Masking screen appears and reports that the CV Status is
DIVERGING, the %CV of the blank wells is higher than the Blank threshold CV%
defined for the plate. Invalid blank wells are highlighted in red. Tap a well to
mask (exclude) it from blank average and %CV calculations. When the
remaining unmasked blank wells are valid, the Approve button illuminates. If
the number of valid blank wells is sufficient for the assay, tap Approve,
otherwise select Cancel and address the problem (e.g., clean the slides).
• At least one blank well is required. If all blank wells are masked, the CV Status is
INVALID.
• Each masked blank well will be flagged with an asterisk in the printed and
exported results.
5. When the read is finished, the results are displayed and ready for output according to
the settings under Instrument > Output.
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