Absorbance Plate Test | 25
Delta 2: 10045 - 10034 = +11
Delta 3: 15630 - 15615 = +15
Delta 4: 21851 - 21835 = +16
MONOCHROMATOR
A = +0.000000 B = -0.000390 C = -0.161696
SYSTEM TEST PASS
0000
Reviewed/Approved By: _______________________________________
Date: ________________
Figure 6: System Self Test report
Absorbance Plate Test
Description
This test uses BioTek’s 7-Filter Absorbance Test Plate (PN 7260522) to confirm the
mechanical alignment; optical density accuracy, linearity, and repeatability; and
wavelength accuracy to NIST-traceable values.
An alternate method that may be used to determine
accuracy/linearity, repeatability, and alignment is Liquid Test 2,
described on page 37.
The Absorbance Plate Test confirms the following:
•
Mechanical Alignment: The Test Plate has several groups of precisely
machined holes to confirm the mechanical alignment of different
microplate readers. The amount of light that shines through these holes is
an indication of whether the reader is properly aligned with the absorbance
optical path. A reading of more than 0.015 OD for any of the designated
alignment holes indicates that the light is being “clipped” and the reader
may be out of alignment.
•
Accuracy/Linearity: The Test Plate contains neutral-density glass filters
of known OD values at several wavelengths. Actual measurements are
compared against the expected values provided in the Test Plate’s
Standards Certificate. Since there are several filters with differing OD
values, the accuracy across a range of ODs can be established. Once it is
proven that the reader is accurate at these OD values, the reader is also
considered to be linear.
•
Repeatability: This test ensures the instrument meets its repeatability
specification by reading each neutral-density filter on the Test Plate twice
with the filter in the same location.
Epoch Operator’s Manual
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