30 | Chapter 4: Instrument Qualification
Repeatability Results
Wells C1 E2 G3 H6 F5 D4
Read 1 0.134 0.566 1.039 1.557 1.864 2.392
Min Limit 0.127 0.555 1.024 1.537 1.840 2.315
Max Limit 0.140 0.577 1.054 1.578 1.888 2.469
Read 2 0.134 0.566 1.039 1.557 1.864 2.390
Result PASS PASS PASS PASS PASS PASS
Wavelength = 690 nm
Accuracy Results
Wells C1 E2 G3 H6 F5 D4
Reference 0.127 0.485 0.881 1.323 1.537 1.972
Min Limit 0.104 0.455 0.843 1.277 1.486 1.913
Max Limit 0.150 0.515 0.919 1.369 1.588 2.031
Read 1 0.125 0.482 0.879 1.319 1.535 1.968
Result PASS PASS PASS PASS PASS PASS
Repeatability Results
Wells C1 E2 G3 H6 F5 D4
Read 1 0.125 0.482 0.879 1.319 1.535 1.968
Min Limit 0.119 0.472 0.865 1.301 1.515 1.943
Max Limit 0.131 0.492 0.893 1.337 1.555 1.992
Read 2 0.125 0.482 0.879 1.318 1.534 1.968
Result PASS PASS PASS PASS PASS PASS
Wavelength = 750 nm
Accuracy Results
Wells C1 E2 G3 H6 F5 D4
Reference 0.134 0.434 0.783 1.179 1.272 1.632
Min Limit 0.111 0.405 0.747 1.135 1.227 1.579
Max Limit 0.157 0.463 0.819 1.223 1.317 1.685
Read 1 0.131 0.432 0.781 1.176 1.273 1.630
Result PASS PASS PASS PASS PASS PASS
Repeatability Results
Wells C1 E2 G3 H6 F5 D4
Read 1 0.131 0.432 0.781 1.176 1.273 1.630
Min Limit 0.125 0.423 0.768 1.159 1.255 1.609
Max Limit 0.138 0.441 0.794 1.193 1.290 1.652
Read 2 0.131 0.432 0.781 1.176 1.273 1.631
Result PASS PASS PASS PASS PASS PASS
Reviewed/Approved By: _______________________________________
Date: ________________
Figure 8: Sample output for the Absorbance Plate Test
Results
• Peak Absorbance: When the test is performed, the C6 filter is scanned at
the test range(s) defined by the user in the Absorbance Test Plate dialog. To
verify wavelength accuracy, the wavelength of the maximum absorbance is
compared with the peak wavelength value entered in the software, which
comes from the Standards Certificate supplied with the Test Plate. The
BioTek
®
Instruments, Inc.
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