Absorbance Plate Test | 31
accuracy of the wavelength should be ± 3 nm (± 2 nm instrument, ± 1 nm
filter allowance).
For example, if the test range is 580 to 590 nm, the Certificate value is 587 nm,
and the reader reports a peak value of 590 nm, then the reader meets
specifications. If the reader reports 591 nm, then the reader does not meet
specifications.
If the reader fails this test:
¾ Make sure the information entered into Gen5 matches the information
on the Test Plate’s Peak Wavelength Certificate.
¾ Verify that the Test Plate actually has a filter in location C6.
¾ Check the C6 filter to make sure it is clean. If needed, clean it with lens
paper.
Do not remove the filter from the Test Plate, and do not use alcohol or
other cleaning agents.
¾ Make sure the Test Plate is within its calibration certification period. The
calibration sticker is affixed directly to the plate. If it is out of date,
contact BioTek to schedule a recertification.
¾ Check the microplate carrier to ensure it is clear of debris.
•
Alignment: This portion of the test measures the alignment of the
microplate carrier with the optical path. A reading greater than 0.015 OD
represents an out-of-alignment condition. Wells A01, A12, H01, and H12
are the only valid alignment holes for the reader on the PN 7260522 Test
Plate.
If the reader fails this test:
¾ Ensure that the Test Plate is correctly seated in the microplate carrier.
¾ Check the four alignment holes (B2, B12, G1, and G11) to ensure they are
clear of debris.
¾ Check the microplate carrier to ensure it is clear of debris.
•
Accuracy: Accuracy is a measure of the optical density of Test Plate wells
C01, D04, E02, F05, G03, and H06 as compared with known standard values
contained in the Standards Certificate that accompanies each Test Plate.
If the reader fails this test, review the following possible problems and
solutions:
¾ Verify that the filter calibration values entered in Gen5 are the same as
those on the Test Plate’s Standards Certificate.
¾ Check the neutral-density filters on the Test Plate to ensure they are
clean. If necessary, clean them with lens paper.
Epoch Operator’s Manual
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