Liquid Testing | 37
Liquid Test 2
The recommended method for testing the instrument’s alignment, repeatability,
and accuracy is to use the Absorbance Test Plate (see page 25). If the Test Plate is
not available, however, Liquid Test 2 can be used for these tests.
Materials
• A new 96-well, clear, flat-bottom microplate (Corning Costar #3590 is
recommended)
• Ten test tubes, numbered consecutively, set up in a rack
• Calibrated hand pipette (Class A volumetric pipette recommended)
• Solution A or B (see the instructions for Liquid Test 1)
• A 0.05% solution of deionized water and Tween 20
Prepare the Dilutions
• Create a percentage dilution series, beginning with 100% of the original
concentrated stock solution (A or B) in the first tube, 90% of the original
solution in the second tube, 80% in the third tube, all the way to 10% in the
tenth tube.
• Dilute using the 0.05% solution of deionized water and Tween 20.
Test Tube Dilutions for Liquid Test 2
Tube Number: 1 2 3 4 5 6 7 8 9 10
Volume of Original
Concentrated Solution (mL)
20
18
16
14
12
10
8 6 4
2
Volume of 0.05% Tween
Solution (mL)
0
2
4
6
8
10
12 14 16
18
Absorbance expected if original
solution is 2.0 at 200 μL
2.0
1.8
1.6
1.4
1.2
1.0
0.8 0.6 0.4
0.2
The choice of dilutions and the absorbance of the original solution can
be varied. Use this table as a model for calculating the expected
absorbances of a series of dilutions, given a different absorbance of
the original solution.
Prepare the Plate
• Pipette 200 µL of the concentrated solution from Tube 1 into each well of the
first column, A1 to H1, of a new flat-bottom microplate.
Epoch Operator’s Manual
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