26 | Chapter 4: Instrument Qualification
• Wavelength Accuracy: BioTek’s Absorbance Test Plate with the part
number 7260522 contains a glass filter in position C6. This filter is used to
check the wavelength accuracy of the absorbance monochromator. The
filter is scanned across a specified wavelength range in 1-nm increments.
The wavelength of maximum absorbance is compared to the expected peak
wavelength supplied on the Test Plate’s certificate and entered into Gen5.
Test Plate Certificates
To run the Absorbance Plate Test on the Epoch, you will need BioTek’s 7-Filter
Absorbance Test Plate (PN 7260522) with its accompanying certificates.
• The Standards Certificate contains standard OD values for the filters at
several different wavelengths. See the sample Standards Certificate in
Figure 7
below.
• The Peak Wavelength Certificate contains one or more “Peak Wavelength”
values for the glass filter in position C6 on the plate. Each value has a valid
test range associated with it. For example, a Peak Wavelength value may be
586 nm with a test range of 580 to 590 nm
(or tolerance values of –6/ +4).
This test plate can be used for testing the reproducibility, linearity, and alignment of your
BioTek autoreader. The following calibration data has been recorded by a NIST-traceable
spectrophotometer.
WAVELENGTH (nm)
Well 405nm 450nm 490nm 550nm 620nm 630nm 690nm 750nm
C1 0.147 0.140 0.135 0.130 0.136 0.136 0.127 0.134
E2 0.618 0.575 0.574 0.568 0.573 0.568 0.485 0.434
G3 1.133 1.052 1.051 1.040 1.050 1.040 0.881 0.783
H6 1.701 1.578 1.577 1.560 1.575 1.560 1.323 1.179
F5 2.279 2.024 1.976 1.956 1.893 1.865 1.537 1.272
D4 2.945 2.604 2.545 2.513 2.437 2.400 1.972 1.632
Set #2453 Serial # 161259
S A M P L E
Figure 7: Sample Standards Certificate, showing OD/Wavelength combinations
for each of six locations on the Absorbance Test Plate
Before the Absorbance Plate Test can be performed, the standard OD values and
the peak wavelength value(s) must be entered into Gen5. This has to be done only
once.
BioTek
®
Instruments, Inc.
To Next Page
Loading...
Need help?
General
