52 | Appendix A: Specifications
Optical Performance
Accuracy, Linearity, Repeatability
All qualifications were conducted using 96-/384-well, flat-bottom
and round-bottom microplates.
For the performance described here, the Gain on the Optics Test should be equal to or less
than 5.0.
Measurement Range: 0.000 to 4.000 OD Resolution: 0.0001 OD
Accuracy
96-well plate, normal read speed
0.000–2.000 OD +/-1% +/-0.010 OD
2.000–2.500 OD +/-3% +/-0.010 OD
384-well plate, normal read speed
0.000–1.500 OD +/-2% +/-0.010 OD
1.500–2.000 OD +/-5% +/-0.010 OD
96-well and 384-well plate, sweep read speed
0.000–1.000 OD +/-1% +/-0.010 OD
Linearity (by liquid dilution)
96-well plate, normal read speed
0.000–2.000 OD +/-1% +/-0.010 OD
2.000–2.500 OD +/-3% +/-0.010 OD
384-well plate, normal read speed
0.000–1.500 OD +/-2% +/-0.010 OD
1.500–2.000 OD +/-5% +/-0.010 OD
96-well and 384-well plate, sweep read speed
0.000–1.000 OD +/-1% +/-0.010 OD
Repeatability (measured by one standard deviation: 8 measurements/data points)
96-well plate, normal read speed
0.000–2.000 OD +/-1% +/-0.005 OD
2.000–2.500 OD +/-3% +/-0.005 OD
384-well plate, normal read speed
0.000–1.500 OD +/-1% +/-0.005 OD
1.500–2.000 OD +/-3% +/-0.005 OD
96-well and 384-well plate, sweep read speed
0.000–1.000 OD +/-2% +/-0.010 OD
BioTek Instruments, Inc.