Dionex RF-2000 - Repeatability Test of Chromatographic Data (Gradient LC)

Operating Instructions RF-2000
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XAXI
SD
XA = (X1 + X2 + ...... + X4 + X5) / 5
%RSD = (SD / XA) * 100
X1 .....X5 : Data
XA : Average
SD : Standard deviation
%RSD : Relative standard deviation
Acceptance Criteria
The acceptance criteria for the %RSD are shown below.
Retention time : 0.5% or less
Peak area : Acridine 5.0% or less, Anthracene 2.0% or less
Peak height : Acridine 5.0% or less, Anthracene 2.0% or less
(It is necessary that changes of room temperature are less than 2°C)
Because the fluorescence intensity of Acridine changes more than that of Anthracene
by the fluctuation of the room temperature, the %RSD of Acridine is larger than that
of Anthracene.
6.3 Repeatability Test of Chromatographic Data (Gradient LC)
6.3.1 Purpose
The purpose of this test is to confirm that the chromatographic data can be obtained
with good repeatability for the LC system to be inspected.
An HPLC System for this inspection consists of the pump, detector, column oven,
auto injector, and data system.
6.3.2 Preparation for Inspection
(1) Prepare the following parts and reagents.
(a) Gradient LC system
(b) Mobile phases
Solvent A: acetonitrile (HPLC grade or equivalent)
Solvent B: water (HPLC grade or equivalent)
(c) Column
(4.6mm ID X 150mm)
(d) Sample
* Mixture of two components (acridine and anthracene)