Dionex RF-2000 - Operating Instructions RF

Operating Instructions RF-2000
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The detector reacts as if a Scan procedure was started. The result of the difference
formation is saved to the RF-2000 spectra library again. This spectrum indicates the
optimum emission or excitation value.
Important:
The basic requirement for forming difference spectra is the
correspondence between the wavelength range defined by the
start wavelength and the end wavelength as well as spectra
type (Excitation or Emission).
PGM-File: Scan Procedure
The following PGM-file allows you to find optimum values for the "Emission"
parameter. Proceed in the same way for the "Excitation" parameter.
-0.1 Pressure.LowerLimit = 0
Pressure.UpperLimit = 400
%A.Equate = "%A"
%A.Type = Automatic
%B.Equate = "%B"
%B.Type = Automatic
%C.Equate = "H2O/MeOH 80/20"
%C.Type = Automatic
%D.Equate = "%D"
%D.Type = Automatic
Flow = 1.000
%B = 0.0
%C = 100.0
%D = 0.0
Emission.ExWavelength = 275
Emission.EmWavelength = 350
Emission.Gain = 1.0
Emission.Response = 0.5
Emission.Sensitivity = Low
Emission.Step = Auto
Emission.Average = On
0.000 Emission.Autozero
Inject
Emission.AcqOn
6.000 ; stop flow
Flow = 1.000
Flow = 0.000
; start scanning
Emission.ScanEmission
; wait for start of scan
Wait Emission.Scanning
; wait for end of scan
Wait Not emission.Scanning
; continue flow
Flow = 0.000
Flow = 1.000
10.000 Emission.AcqOff
End