Operating Instructions RF-2000
55
(6) Calculate the average (X) of the data and %RSD using the equations as
shown below.
4
)(
5
1
2
å
=
−
=
i
XAXI
SD
XA = (X1 + X2 + ...... + X4 + X5)/5
%RSD = (SD/XA) *100
X1.....X5 : Data
XA : Average
SD : Standard deviation
%RSD : Relative standard deviation
Acceptance Criteria
The acceptance criteria for the %RSD are shown below.
Retention time: 0.5% or less
Peak area : Acridine 5.0% or less, Anthracene 2.0% or less
Peak height : Acridine 5.0% or less, Anthracene 2.0% or less
(It is necessary that changes of room temperature are less than 2°C)
* Reference: Ternary or quaternary gradient system is inspected in basically the
same way.
Because the fluorescence intensity of Acridine changes more than that of Anthracene
by the fluctuation of the room temperature, the %RSD of Acridine is larger than that
of Anthracene.
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