Installation and commissioning PlasmaQuant MS product family
52
Always change the sample and Internal Standard tubing at the same time to avoid
uneven wear of the tubing.
1. Fit another pump tubing to the peristaltic pump in the next available channel.
This will be the Internal Standard channel. The pump tubing should be the same
type as the sample tubing. Ensure that the pressure bar is adjusted as per the
sample tubing.
2. Connect the outlet of the Internal Standard channel to a commercially available
plastic Y-piece (or T-piece). Connect a piece of capillary tubing to the inlet end of
the Internal Standard tubing. The capillary can then be placed in a suitable vessel
(e.g., a 500 mL container) containing the Internal Standard solution.
3. Connect the outlet of the sample pump tubing and the nebulizer capillary tubing to
the other arms of the Y-piece/T-piece. In this way, the Internal Standard will be
mixed with the sample at the Y-piece/T-piece and the combined flow will pass to
the nebulizer.
tubing connector
Y-piece
Pump tubing for sample
and Internal Standard
with color coding ‘black-
black‘
Capillary tubing - to be
placed in the sample and
Internal Standard solution
Fig. 23 Pump tubing with Internal Standard tubing
Y-piece
Sample tubing
Internal Standard
tubing
Drain tubing from the
spray chamber
Fig. 24 Peristaltic pump - tubing connections with Internal Standard