Connect an empty column
Follow the instruction below to connect an empty column to ÄKTAprocess.
ActionStep
Connect tubing between the system valve marked COLUMN BOTTOM and
the column bottom.
1
Connect tubing between the system valve marked COLUMN TOP and the
column top.
2
Connect a packed column
without bypass lines/valves
Follow the instruction below to connect a pre-packed column without by-pass lines/valves
to ÄKTAprocess.
ActionStep
Connect tubing to the system valve marked COLUMN BOTTOM, but do not
connect the other end of the tubing to the bottom of the column at this time.
1
Set the system column valves to the UpFlow position.2
Using the pump, fill the system with an appropriate liquid for column instal-
lation.
3
When the system, including the tubing connected to the column bottom
system valve, is filled with liquid, connect the other end of the tubing to the
bottom of the column.
4
Connect tubing between the top of the column and the system valve marked
COLUMN TOP.
Note:
If the column will be run in downwards mode after the connection, the tubing
connected to the column top must also be filled with liquid before it is connect-
ed to the column top.
5
ÄKTAprocess Operating Instructions 29-0252-49 AA 103
4 Installation
4.4 ÄKTAprocess Setup
4.4.6 Connect a column
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