Connect a packed column with
bypass lines/valves
Follow the instruction below to connect a pre-packed column with bypass lines/valves
to ÄKTAprocess.
ActionStep
Connect tubing between the column bottom and the system valve marked
COLUMN BOTTOM.
1
Connect tubing between the column top and the system valve marked
COLUMN TOP.
2
Set the system column valves to the UpFlow position.3
Using the pump, fill the system with an appropriate liquid for column instal-
lation.
4
When the system, including the tubing connected to the column, is filled
with liquid, stop the pump.
5
Use the manual valves on the column to change from bypass to in-line.6
Final considerations
Once the column is connected, the following should be taken into consideration.
•
Make sure to activate the system alarm(s) again after the filling sequence.
•
When the pump is started, the system must be in Column UpFlow position.
104 ÄKTAprocess Operating Instructions 29-0252-49 AA
4 Installation
4.4 ÄKTAprocess Setup
4.4.6 Connect a column
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