Start a run
Follow the instruction below to start a run.
ActionStep
In the System Control module, select Run in the File menu.1
Select the method to start. Click OK.2
A Start Protocol is displayed, consisting of a number of dialog boxes. On the
Variables page, it is possible to fine-tune the method before proceeding.
Checking the Show details box will display more detailed information.
Check that the sample volume is correct.3
Click Next or Back to navigate through the dialog panels, adding the infor-
mation required as well as your own comments.
4
Click Start in the Result Name dialog box. This will initiate the method run.5
Monitor the run
During the run, the System Control module will display the run progress of the method
being executed.
To interrupt a method during a run you may use the Hold, Pause or End icons in System
Control. A held or paused method run can be resumed by using the Continue icon. See
the instructions in the table below.
then...If you want to...
click the Hold icon.temporarily hold the method, with current flow
rate and valve positions sustained
click the Pause icon.temporarily pause the method, and stop all
pumps
click the Continue icon.
Note:
An ended method cannot be contin-
ued.
resume, for example, a held or paused method
run.
click the End icon.permanently end the run
When ending a method run prematurely, it is possible to save the partial results.
Note:
More information regarding UNICORN capabilities during the method run is available in
the UNICORN user documentation.
ÄKTAprocess Operating Instructions 29-0252-49 AA 125
5 Operation
5.2 Perform a run