- Volume is displayed (41) in ml.
- Desired volume can be selected with arrow keys (35).
- At this point the processor is going to determine the number of revolutions required.
- Since every tube has its own tolerance, it will be required to calibrate the pump drive for a
particular tube, if high accuracy is mandatory.
9.3.6. Calibrate metered volume
- Determine and set speed by your actual requirements
The PD 5206 drive can only meter with a maximum of 300 l/min for reasons of
precision! During the metering process, the drive is automatically limited to 300 l/min.
The LED "Speed rpm" and the display blink before the metering process as an
- Enter tube data and volume to be metered, as described in chapter 10.3.5.
- Hit "Start Stop" key (32).
- Pump is going to feed fluid at the speed and volume entered before.
- Determine exact quantity of fluid collected.
- Hit "Cal" key (34). Display (41) flashes.
Caution: with reference to the very small quantities involved, feed rate is displayed in
µl/min, when Cassette small and tube size 0.2; 0.5 or 0.9 are selected.
- Enter value so determined with arrow keys (35).
- Hit "Cal" key (34) and hold depressed for not less than 3 seconds to enter. Data in memory
are revised. "Cal" is displayed (41) for about 2 seconds to confirm action.
- Enter volume wanted with arrow keys (35).
Programmed flow rate and hence volume metered are a function of pump head and
tube selected. If, during calibration, the user finds a deviation in excess of 30 %, a
setting error is very probable. In this case discontinue calibration (hit "select" key once),
and go ahead checking the following parameters:
- did you select the right pump head (chapter 10.3.1)?
- did you choose the right tube (chapter 10.3.3)?
- is the tube properly installed in the pump head (chapter 3) ? Is the clamping action
If wrong parameters have been entered for calibration of metered volume, metering
action cannot be started with the "Start" key. The "Select" will turn to "Flow ml/min",
and 4 dashes (----) are displayed.
Correct wrong parameters as described below:
- go into feed rate graph (chapters 17 & 18) and determine a speed figure (1/min)
plus a logical feed rate (ml/min).
go to "speed" menu (1/min) with "Select" key and choose a speed with arrow keys.
- go to "flow" menu (ml/min) with "Select" key and choose a feed rate from the graph,
as described in chapter 10.3.4.
at this point a reasonable feed rate is saved.
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