Hitachi CP100NX User Manual

3-2-2 Acceleration and Deceleration Modes
In order to meet a wide variety of applications, the acceleration and deceleration rates can be adjusted
between 0 to 500 rpm. By setting an ideal acceleration and deceleration time samples will not be
disturbed, which improves efficiency and reduces centrifugation time.
The table below shows the relationship between ACCEL and DECEL code numbers and acceleration
and deceleration time.
*1 Minimum time: The time it takes the drive motor to accelerate and decelerate the rotor using
maximum torque.
This time will vary with rotor type and the mechanical resistance of the drive motor.
*2 DECEL code "F": A deceleration method that does not involve brake control using the drive motor.
The deceleration time will then vary widely depending on differences in the small
mechanical resistance of the drive motor and the vacuum level of the rotor chamber
and other factors.
[Examples showing use of acceleration and deceleration modes)
Optimum
numeric code
Separation characteristics
ACCEL
DECEL
Density gradient
centrifugation using a
vertical rotor
5 7
As the density gradient reorients during
acceleration and deceleration, the gradient may be
disturbed if samples are rapidly accelerated and
decelerated.
Using CsCl
density-gradient
sedimentation equilibrium
centrifugation for DNA
separation (when using a
uniform liquid)
9 7
You can operate at maximum acceleration because
the density gradient is not formed during the run.
Slow deceleration is recommended to obtain sharp
bands.
Pelleting using an angle
rotor
9 9
Rapid unloading of samples is possible.
(short separation time)
Density gradient
centrifugation using swing
bucket rotor
8 8
The sample and gradient do not reorient. For that
reason, there is less gradient turbulence than with a
vertical rotor and good results can be obtained as
long as rapid acceleration or deceleration are
avoided.
With a swinging bucket rotor, there is little difference with regard to turbulence if
ACCEL/DECEL is less than or equal to 8. However, rotor swinging may become large in
modes with long acceleration times, which could cause an imbalance alert to light even
within tolerable imbalance values.
Code
No.
Acceleration
(minutes) from 0
to 500 rpm
Deceleration from
(minutes) from
500 to 0 rpm
9
8
7
6
5
4
3
2
1
0
F
(*2)
Minimum time
(*1)
1
2
3
4
5
6
7
8
9
－
Minimum time
(*1)
1
2
3
4
5
6
7
8
9
Free coast from
stable speed
3-2-2 Acceleration and Deceleration Modes
NOTE
Indicates ACCEL and
DECEL code numbers
(Free coast)
Time
Speed
(rpm)
3-10

Hitachi CP100NX Specifications

General

Brand	Hitachi
Model	CP100NX
Category	Laboratory Equipment
Language	English