NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
48
BASIC OPERATION
The NanoPhotometer
®
product line offers a complete solution for NanoVolume
(N50/N60/NP80/N120/C40) and standard cuvette volume (C40/NP80) applications. With the
NanoPhotometer
®
N50/N60/NP80 NanoVolume applications the sample volume ranges from
0.3 µl to a maximum sample volume of 2.0 µl. For the NanoVolume applications with the
NanoPhotometer
®
N120 a minimum of 2 µl sample volume is necessary. Standard cuvette
applications can be performed with quartz, glass or plastic cuvettes with a pathlength of 10
mm, 5 mm, 2 mm, 1 mm or 0.5 mm and a center height of 8.5 cm.
Note: NanoVolume application in the NanoPhotometer
®
C40 is only available with the
optional accessory “submicroliter cell” from Implen.
NANOVOLUME SINGLE SAMPLE MEASUREMENT BASICS (N50/N60/NP80)
1. Select a method depending on your sample and set the parameters for the
measurement.
2. Ensure that the sample window on pedestal and the mirror in the lid arm are clean.
3. Use the integrated vortexer (N60 and NP80 only) to mix the sample to achieve a
homogenous sample
4. Raise the lid arm and pipette the appropriate amount of blank solution onto the
illuminated sample window on pedestal. The Illumination turns automatically off when
the lid arm is lowered.
Note: Do not overfill the well.
Note: The low energy red light (LED) illumination can be switched of in Preferences
5. Lower the lid arm and initiate a blank measurement with the blank button
6. Clean the measurement window and mirror on the lid arm with a slightly wet lint-free
tissue. Use water, 70% ethanol or isopropanol if needed.
To Next Page
Loading...
Need help?
General
