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NanoPhotometer

N120/NP80/N60/N50/C40 User Manual Version 4.3.1

Note: Auto sample function is only available NanoVolume methods and

new NanoPhotometer

versions it may not be available after updating an

older firmware version.

MEASUREMENT PROTOCOL MULTI SAMPLE (N120)

1. Select the Nucleic Acids icon on the home screen.

2. To change between single and multi-sample application use the toggle

switch Single Sample in the parameter area.

3. Parameter setting Nucleic Acid type:

To change the nucleic acid type, select either dsDNA, ssDNA, RNA, miRNA, miRNA

Sequence, Oligo, Oligo Sequence or Custom (see Table 1 on page 66).

• The miRNA sequence and Oligo sequence options allow the sequence to be entered

and the extinction coefficient will be automatically calculated. A minimum of 4 bases is

required.

• The default setting for Custom is 50. The extinction coefficient (1/Ɛ) can be set in a

range of 15 - 150. It is possible to enter a name for the custom nucleic acid factor for

documentation.

4. Select the Units in which the concentration should be calculated. Options are ng/µl, µg/µl

and µg/ml. pmol/µl if a nucleic acid sequence is entered for nucleic acid factor calculation.

5. The background correction is enabled at 320 nm by default. It is possible to disable the

background correction with the toggle switch. Selection options are 320 nm, 340 nm. It is

Implen NanoPhotometer NP80 User Manual

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