NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
104
10. Use a lint-free laboratory wipe to clean both the sample window on
pedestal and mirror in lid arm prior to applying the next sample.
Note: It can be helpful to apply the blank a second time and read it as a
sample to ensure a proper blank.
11. Apply sample to the sample window on pedestal and press the sample
button to initiate the measurement.
If auto sample is active the sample measurement is automatically
started after closing the lid arm.
Note: Auto sample function is only available NanoVolume methods and
new NanoPhotometer
®
versions it may not be available after updating
an older firmware version.
CALCULATIONS
Formula Calculation:
Is depending on the entered formula in the parameter concentration.
Absorbance Calculation:
Absorbance is formally defined as the decimal logarithm (base 10) of the reciprocal of
transmittance:
A= log
1
T
=- log T
T=10
(-A)
Note: Corresponding absorbance value e.g. Absorbance value ( = 230) etc. normalized to
10 mm path length
%Transmittance Calculations (cuvette mode only)
In the wavelength application it is possible to measure the absorbance (A) and %
transmittance (%T) of a sample with respect to a reference at a specific wavelength.
Transmittance is the ratio of light intensity remaining after it has passed through the sample
(I) to the initial incident light intensity (I
0
):
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