NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
96
6. Insert cuvette with the reference sample and select blank button to initiate
the measurement.
7. Insert cuvette with the sample and select the sample button to initiate the
measurements. Once the kinetic is started the Blank button turns to a
Pause/Continue button and the Sample button to a Stop button.
Note: While the kinetics is running it is not possible to change the parameters, save data or
delete data. Change of parameters is only possible before starting the kinetic readings. Save
and delete data is only available after the kinetic session is stopped.
Note: Auto print and cryo label print is not available in Kinetics.
CALCULATIONS
All absorbance values are normalized to a 10 mm path.
A
0
= absorbance of start value (10 mm path)
A
n
= absorbance of actual value time n (10 mm path)
dA = absorbance of actual value – absorbance of start value
Slope = linear regression fit of all actual measurement points
Final A = absorbance of final value
R
2
= R
2
=
(O
i
-E
i
)
2
E
i
n
i=1
[O
i
= observed slope value; E
i
= expected slope value]