NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
54
highly concentrated samples or certain proteins, the recommended procedure for
cleaning is to use a slightly wet lint-free laboratory wipe (with water or 70% EtOH
depending on sample type) to thoroughly clean the sample surface.
It is mandatory that the metal contact face around the measurement window and the
mirror is clean.
NanoVolume Methods (N120)
The sample windows on pedestal are illuminated with a low energy red light to assist
with accurate sample application. Depending on selected samples, the red lights are
turned on for measurement spots, which will be measured. Apply samples on illuminated
measurement spots.
The red light is switched off once the lid arm is closed. It is possible to disable the
illumination feature in preferences of the NPOS software.
e.g. 12 samples selected:
For blank and sample measurements 2 µl of sample volume are necessary. Pathlength
will be set automatically, if necessary.
Proper cleaning is important to ensure accurate measurements. In most cases a dry lint-
free laboratory wipe is sufficient to clean the sample quartz surfaces. In the case of
highly concentrated samples or certain proteins, the recommended procedure for
cleaning is to use a slightly wet lint-free laboratory wipe (with water or 70% EtOH
depending on sample type) to thoroughly clean the sample surface.
It is mandatory that the metal contact face around the measurement window and the
mirror is clean.
Close the lid arm carefully. Do not push the lid arm down forcefully. Do not touch the lid
arm during measurements.
Cuvette Methods
The cuvette holder (NP80/C40 only) is compatible with standard 10 mm path length
quartz, glass and plastic cuvettes with an optical height of 8.5 mm.
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