NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
82
W/o background and with dye correction:
DOL =
A
max, dye
* ε
prot
A
280
cf
dye
* A
max, dye
* ε
dye
W/o background and w/o dye correction:
DOL =
A
max, dye
* ε
prot
A
280
* ε
dye
DOL Degree of labeling/dye per protein ratio
A
max,dye
Absorbance value at the absorbance maximum of the dye (10 mm path)
A
280
Absorbance at 280 nm (10 mm path)
A
BKG
Absorbance at selected background wavelength (10 mm path)
ε
dye
Extinction coefficient of dye in M
-1
*cm-
1
ε
prot
Extinction coefficient/protein factor in g*cm/l
cf
dye
Dye-dependent correction factor at 280 nm
Table 4. Dye Types, Absorbance Max, Extinction coefficients, and dye-dependent
correction factors
Absorbance
maximum
of Dye (nm)
Dye-dependent
ext. coeff.
ε
dye
in M
-1
*cm
-1
Dye-dependent
correction
factor at 280 nm
cf
dye
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