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Implen NanoPhotometer NP80 - Page 85

Implen NanoPhotometer NP80
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NanoPhotometer
®
N120/NP80/N60/N50/C40 User Manual Version 4.3.1
85
MEASUREMENT PROTOCOL SINGLE SAMPLE
1. Select protein assays icon from home screen.
2. To change between NanoVolume and cuvette application, use the
Change to Cuvette/NanoVolume button below the parameter area
(NP80/C40 only).
3. To change the assay type push on Bradford and a list with available options opens on the
right side.
Options are: BCA Assay, Biuret Assay (cuvette only), Bradford Assay, Lowry Assay (not for
N50)
4. For Cuvette Application:
Select the path length depending on the used cuvette.
Options are: 0.5 mm, 1 mm, 2 mm, 5 mm and 10 mm
If it is desired to heat the sample to 37°C use the toggle switch to turn on cell holder heater.
When the cuvette holder has reached 37°C the toggle color changes to green.
Note: Only available for cuvette applications (NP80 and C40).
For NanoVolume Application:
Select the dilution depending on the sample concentration
Note: There is no automatic path length setting available in this method. Select either a
virtual dilution of 15 (path length 0.67 mm) or of 140 (path length 0.07 mm) depending on
your sample concentration.

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