Implen OD600 - Calculations

Implen OD600
®
User Manual Version 1.0
20
Insert cuvette/tube into the cell holder.
Initiate a sample measurement with the sample button. Upon completion of measurement remove the cuvette.
Apply further samples.
Note: Handling Out-of-Range Measurements - If a sample measurement is out of range (>4A), a warning popup will
appear, instructing you to repeat the measurement and dilute the sample accordingly.
Note: If the light path is blocked by an obstacle, the error code Err4 will appear - Please remove the obstacle from the light
path to proceed with the measurement.
4. Saving and Accessing Measurements
All sample measurements and sets are automatically saved and can be accessed through the Sample List feature in
the respective app. Data can be exported through the sample list function using a USB-Stick.
Calculations
OD600 = A
600
* Ð
OD600 Optical density at 600 nm
A
600
Absorbance at 600 nm (10 mm path)
Ð Dilution factor
Cells ml
⁄
= A
600
* Ð * cf * multiplier
A
600
Absorbance at 600 nm (10 mm path)
Ð Dilution factor
cf Correction factor for spectrophotometer
multiplier Multiplier of sample