Implen OD600
®
User Manual Version 1.0
37
6. Troubleshooting
Note: the technical notes can be found on implen.de
A flashing Absorbance
reading of 4.00 Abs is
obtained.
This indicates an Absorbance of more than 4.00 and is therefore out of range. The sample
needs to be diluted or use DiluCell
TM
10 for virtual dilution.
A negative reading is
obtained.
In normal measurements the test sample has a positive Absorbance compared to that of
the Reference. Negative readings will be obtained if the Reference and Test cuvettes are
mixed up.
A flashing Absorbance
reading of –0.30 Abs is
obtained.
This indicates an Absorbance of less than –0.30 Abs and is therefore out of range. You
need a higher concentrated sample.
Please also ensure that your Blank measurement was performed with a suitable blank
solution.
Unexpected results are
obtained.
Any bubbles in solution will produce considerable error. Ensure the sample is free of foam
or bubbles and repeat the measurement.
The OD600 value is
different to that
obtained on another
instrument in the lab.
When you measure turbid solutions you do not measure the absorbance/transmittance
of light at the detector, you measure the amount of scattered light that reaches the
detector. Thus optical geometry is very important - the further the distance from the
sample to the detector, the greater the effect of the scattered light. Thus instead of
harvesting at 0.4 OD, for example, you have do it at 0.8 OD. A simple conversion factor
can be calculated from the OD 600 of your existing instrument compared to that of the
Implen OD600 instrument. For further information see also Technical Note #8 OD600.
Note: the technical notes can be found on implen.de
Device shuts down
and restarts after ESD-
impulse
If the device shuts down after an ESD-impulse, it will restart and move back into the home
screen.
Device shuts down
and restarts: Light
Path Obstruction Error
If the device crashes and a sample is still inside, the light path for the self-test is blocked.
Solution: Remove the sample to clear the light path, then blank again.
The USB Stick is not
detected by the device
If the USB stick is not working, please re-format it to FAT32 using a Windows PC.
The selected data was
not exported
In the sample list screen, only the four samples in the visible screen can be selected at a
time. If yu scroll to the next page, the samples from the previous page will be unselected. If
you want to export all data, click on ALL.
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