Implen OD600 - Dilucell Technology; Method Overview

Implen OD600
®
User Manual Version 1.0
28
DiluCell™ technology
Method Overview
The DiluCell™ is a disposable cuvette designed for virtual dilution of cuvette-based samples, utilizing the principle of
the Lambert-Beer law.
Beer-Lambert-Law
Abs = measured absorbance value
I
0
= incoming light
I = outgoing light
ε = extinction coefficient
c = concentration
d = path length (standard 1 cm / 15 mm center height)
Operating instructions
Pipette your reference or sample into the DiluCell
TM
. Place the DiluCell
TM
into the cuvette sample compartment.
Ensure the correct positioning of the DiluCell™. The light beam of the Implen OD600
®
is directed from the front of
the instrument to the back (as indicated by the arrows on the device). The required sample volume is 300 µl.
Note: DiluCell™ will not work in any other orientation; do not mix other cuvette types within a DiluCell™ measurement
series (for blank, etc.).
Note: It is recommended to check for air bubbles before the measurement. In case of an air bubble in the cell please
remove them by gently tapping the cell.
Note: Remember to multiply the reading from the Implen OD600
®
after the measurement with the proper virtual dilution
factor: Virtual dilution factor is: 10 for DiluCell™ 10.
Note: It is not recommended to reuse DiluCell™ due to: high probability of cross contamination and high probability of air
bubbles when refilling the cell.
Note: Do not autoclave the DiluCell™.
Note: The DiluCell™ is approved only for the usage with the Implen OD600
®
.
Note: For solvent compatibility please contact your local Implen partner or the Implen Team at www.implen.de.
DiluCell
TM
10 Specifications
Material
PMMA
Wavelength Range
340 nm - 950 nm
Volume Requirement
300 µl
Pathlength (at 8.5 mm center height)
1 mm
Tolerance
+/- 10%
Ordering Information (pack with 96 cells)
Article No.: DC 10
dc
I
I
Abs  
0
log