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Loading Materials and Equipment
1. Only load the materials required for the procedure. Do not overload the cabinet.
2. Do not obstruct the front, side, or rear return air grilles.
3. Large objects should not be placed close together.
4. Slowly close the sash until it is in the correct operating position.
5. After loading the cabinet, wait two to three minutes to purge airborne
contaminants from the work area.
Work Techniques
Keep all materials at least 4 inches (100 mm) inside from the sash, and perform
all contaminated operations as far to the rear of the work area as possible.
Segregate all clean and contaminated materials in the work area.
Arrange items to minimize movement of contaminated materials into clean areas.
Keep all discarded contaminated material to the rear of the work area.
Avoid moving materials or the operator's hands and arms through the front
access opening during use.
Avoid the use of an open flame. Use disposable labware or an electric incinerator
as alternatives.
Use proper aseptic technique.
Avoid techniques or procedures that disrupt the airflow patterns of the cabinet.
If there is a spill or splatter during use, all objects in the cabinet should be
surface decontaminated before removal. Thoroughly disinfect the working area of
the cabinet WHILE IT IS STILL IN OPERATION, to prevent the release of
contaminants from the cabinet.
Final Purging
Upon completion of work, the cabinet should be allowed to operate for two to
three minutes undisturbed, to purge airborne contaminants from the work area.
Unloading Materials and Equipment
Objects in contact with contaminated material should be surface decontaminated
before removal from the cabinet.
All open trays or containers should be covered prior to removal from the cabinet.
Wipe Down
1. Wipe down the interior surfaces of the cabinet with a suitable disinfectant, or 70%
ethanol, and allowed to dry.
2. Periodically lift the work surface and wipe down the area beneath it.
3. Inspect and clean the towel catch located at the rear of the work area, beneath
the work surface.
4. Dispose of rubber gloves appropriately, and have lab coat laundered properly.
5. Wash hands and arms thoroughly with germicidal soap.
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